Realize your dream project with mRNA

Custom mRNA Synthesis

of the highest quality

made easy & affordable by the mRNA experts at RiboPro
 

 

 

 
Direct to Online Ordering 
Order via our RiboProfessionalsThe contents of our Service explained  Learn how mRNA accelerates your research  

        Or scroll down for more info

We make your mRNA project a success

HIGHEST QUALITY mRNA

No dsRNA, a high capping efficiency and a well-defined A-tail.
Quality you can rely on.

FASTEST TURN-AROUND

Receive your custom mRNA product within 2-3 weeks. We keep you updated all the way.

SUPER SUPPORT

Our support team is here to help you with the entire process. From start to finish; we got you covered.

SENSIBLE SCALES

Start small at ng-scale and be sure we can support you to gram-scale. Always with constant quality.

0x more protein on average with our optimizations
0% of orders are delivered within 3 weeks from start
0+ people dedicated to your success
0ug is our minimum ordering amount for custom mRNA

RiboPro: your provider for any mRNA project

If you are looking for a trust-worthy, experienced source of synthetic messenger RNA, you have come to the right address. We have more than 10 years of experience in mRNA synthesis and several years of experience in translating the needs of our customers into successful mRNA designs. RiboPro's custom mRNA synthesis provides transfection-ready mRNA for your experiments. We are able to provide you with as little as ng-scale for initial experiments, but also up to gram-scale for large projects. To be able to provide continuous service from bench-to-bedside, we are currently setting up GMP manufacturing and aim to be ready for orders in mid 2022.

In any case, the design, amount and other specifics will, of course, be based on your needs and preferences. Lengths up to 15k nt are possible and we offer many modification and purification options to match your experimental/medical requirements.

How to get a quote?

Tells us via our Quotation form exactly what you want and you will receive an initial quotation as soon as possible. In the form you can also add questions, remarks and ask our RiboProfessionals™ to contact you to help make adjustments to your mRNA-design/mRNA-project. Filling out the form is fast, easy and prepares our people to support you the best they can!

How to order mRNA?

We offer 2 easy ways to order custom mRNA:

Consult with a RiboProfessional™
or
Get a quotation via our online mRNAssembler™

Either way; we are here to support you!

How fast can you deliver?

For a typical sequence, you will receive your order within 2-3 weeks. If you provide us with a suitable template, it may even be faster, because 3rd party de novo DNA synthesis is the most time-consuming step of our RNA synthesis process. Due to COVID-19 related busyness at our DNA suppliers, an additional week may be required from time to time.

Our commitment to you

  • Fast-delivery

    Time is of the essence in science and drug development, and we work hard not to waste yours. So, we often start synthesis directly after your OK on the quotation, giving you some time to arrange the PO number internally. This ‘at-risk’ manufacturing is uniquely available at RiboPro. Re-ordering synthetic mRNA is also quick and easy; we keep the DNA from previous orders on file for an even quicker turn-around time.

  • Customer-friendly IP-options

    RiboPro uses only canonical nucleotides, meaning that any sequence requested via the mRNAssembler™ PRO is unencumbered in terms of IP by our synthesis methods. If you requested our patent-pending sequence optimization method to de-immunize the mRNA, our terms and conditions allow you free use for non-commercial research & development. Licenses are available for commercial use.

  • Affordable

    When you ask RiboPro to make the best choices for your experiment, we always keep your budget in mind. We never recommend anything more than you need, and we never include any ‘fancy’ but meaningless options. You can trust us to get you the best custom synthetic mRNA for the best price. Another way we keep prices low, is through constant innovation and lean manufacturing methods.

More reasons to choose RiboPro

mRNA DE-IMMUNIZATION

RiboPro's patent-pending method to de-immunize mRNA is equivalent to or better than chemically modified nucleotides in preventing pro-inflammatory cytokines and enhancing protein expression.

IP-SOLUTIONS

Because we only use our own IP to create your mRNA and/or LNP-formulation, we can provide you with a clear and affordable route to the market and are happy to support your commercial ambitions.

FLUORESCENT-LABEL mRNA

We have 11 colors to fluorescently label any mRNA, including both your custom mRNA and our reporter mRNAs. Ideal as control, or for uptake and bio-distribution studies.

COMPLEMENTARY PRODUCTS

In addition to the synthesis of custom synthetic mRNA, we offer formulation services, RNAse testing and more to support your mRNA research.

The RNAssembler™ STARTER

for those who want RiboPro’s mRNA experts to make the hard choices.
Based on your experimental/medical needs, they will design the best (m)RNA for you. Including the selection of the best 5′ and 3’UTR, A-tail-length, capping type and capping method and more.

The RNAssembler™ PRO

for those who have figured out exactly what they want.
We will synthesize exactly what you asked for. You may include your own 5′ and 3’UTR or choose any of our library. In addition, you have a wide range of modifications and purification options to choose from.

What happens next?

After your quotation/order request

Once you send the form in, we take the following steps a.s.a.p. to help you get the best mRNA as fast as possible:
1. a RiboProfessional™ checks your request,
2. gets in touch if needed, and
3. sends you a quotation within 24h
4. when you OK the quotation, we get started.

For repeat orders (an option in our ordering menu), you will still get a quotation, but we often already start with synthesis ‘at risk’ from DNA that is ‘on file’ to realize the shortest possible turn-around time. Of course, you may still ask for amendments of the order based on the quotation. The risk is entirely ours.

After your order

During production, we do the following things:

  • Optimize the sequence if desired
  • Communicate optimized sequences back to you (optional)
  • Order DNA if needed
  • Perform RNA synthesis
  • Perform Quality Control
  • Arrange shipping
  • Check if material has arrived complete and correct
  • Send invoice
  • Follow-up to help you with your experiments if needed

For planning purposes, you will receive updates at steps 2, 4 and 6.

Our complementary services

SEQUENCE ENGINEERING

RiboPro's patent-pending sequence engineering method to de-immunize mRNA is equivalent to or better than chemically modified nucleotides in preventing pro-inflammatory cytokines and enhancing protein expression.

LNP FORMULATION

A successful mRNA experiment requires good transfection into your cell of choice. We can formulate any mRNA into our LNPs, to do exactly that. Our LNPs are highly efficient in mRNA delivery, low tox and can be easily post-modified.

CRO SERVICES

We offer various mRNA-related contract research services, such as mRNA activity testing, mRNA uptake & bio-material release testing, as well as a whole host of other in vitro assays. Your mRNA never has to leave our facility for great results.

RNAse TESTING

As supplier of synthetic mRNA, we know as no other how important it is to avoid RNAse contamination. But how to verify? With our RNAse-activity testing service, you will have peace of mind that your labware/solutions are clean.

More information to help you choose

  • Why not synthesize mRNA yourself?

    Of course as a life-science researcher you have the knowledge and tools to produce any bio-molecule yourself. However, several issues make this difficult for (m)RNA.

    The disadvantages of self-synthesis of (m)RNA

    So also for the synthesis of mRNA; the multi-step enzyme-based reaction, with 1 or more purifications, in addition to acquiring a suitable DNA template, can be time-consuming; taking 2-3 days per synthesis.

    In addition, especially when a limited amount or number of mRNAs is required, the up-front cost for the reaction ingredients, the quality control, the certified RNAse-free buffers and consumables is high.

    But perhaps more than the reasons listed above, very few labs are well-equipped to work with such a delicate molecule, which is subject to degradation to omni-present and hard-to-destroy RNAses. Working with mRNA as a final product in an experimental setting is certainly doable, but multi-step manipulations during synthesis in a sub-optimal environment present a significant risk to the integrity of the final product.

    And finally, designing a high-performance mRNA requires very specific knowledge on 5’UTR and 3’UTR functioning, secondary structure formation of RNA, the induction of innate immunity by RNA, codon optimization, ideal A-tail length, etc.

    Why choose RiboPro custom mRNA synthesis instead?

    By ordering your desired mRNA from RiboPro’s custom mRNA synthesis service you can get started with your experiment as quickly as possible, without hassle! No need to worry about RNAse contamination during production, no need to setup complex quality control, no need to invest in the equipment and materials; we take care of it all. Our RNA experts make sure you get the best results.

    Order now with the RNAssembler™

  • What is included in the service?

    RiboPro’s custom mRNA service is very flexible; we include as much or as little as you need.

    Generally speaking, we have 2 flavors:
    A. You provide us with a (semi)suitable template, which we use as input for the RNA synthesis. Minor changes to the template to make it suitable are no problem for us.
    B. You provide us with the desired sequence, and we perform de novo DNA synthesis to obtain a suitable template for the RNA synthesis.

    What is included?

    So, in the full-service variant (B) the following is included:
    1. mRNA design and optional sequence optimization
    2. de novo DNA synthesis
    3. DNA prep
    4. RNA synthesis
    5. RNA capping (up to 99% capped, and Cap1 modification possible)
    6. A-tailing to desired length (up to 500nt)
    7. 2 purification round
    8. (optional) dsRNA removal procedure
    9. Quality control (length, sequence verification (DNA), A-tail length, purity)
    10. (optional) Aliquotation
    11. Packaging & shipping
    12. After-care to ensure success in your experiments

    This very complete package is available from €660 + shipment cost (typ. €50-€150) + DNA synthesis (typ. €100-€200)

    Order now with the RNAssembler™

  • How does RiboPro optimize mRNA?

    The performance of a messenger RNA, to be added to cells via standard transfection methods, greatly depends on the design of the sequence, the quality of the synthesis, chemical modifications of specific bases and the purity of the end-product. Introducing a sub-optimal mRNA into cells is known to trigger the innate immune response; resulting in lower protein expression, cellular toxicity and the release of pro-inflammatory cytokines.

    High performance mRNA

    For the best performance, we recommend the following combination of features to be selected for your custom mRNA:

    • 5’UTR: no upstream ATG, kozak or similar present directly upstream of the start codon. For example RiboPro catalogue 5’UTRs.
    • 3’UTR: selected from mRNAs known to be extra-ordinary stable.
    • Cap1 – 95%.
    • 120-150nt A-tail.
    • dsRNA reduction.

    These parameters will provide you with a decent expressing mRNA. However, for any of the following situations:
    • therapeutic applications,
    • a demand for high protein-expression,
    • experiments incompatible with pro-inflammatory cytokine release,
    • or any other application requiring the best protein expression and the lowest toxicity,
    we recommend additional mRNA-specific sequence-optimization. Using RiboPro’s patent-pending technology allows for 5x more protein (as high as 40x more in specific applications) and significant reduction in pro-inflammatory cytokine production.

    If you like to understand why these settings lead to the best performance, read more below.

    The trouble avoided by RiboPro’s high-quality RNA synthesis

    Synthesizing a high-performance messenger RNA requires optimization all through-out the multi-step synthesis, including specialized purification.

    dsRNA

    First of all, during the enzymatic production process, dsRNA can be formed. dsRNA is one of the main triggers of innate immunity, primarily via TLR3 (endosomal recognition during transfection), OAS1, PKR and MDA5 (cytoplasmic sensors). RiboPro’s mRNA synthesis process results in the lowest amount of dsRNA possible. The method used to reduce the amount of dsRNA, also negatively affects the yield of ssRNA, so limited additional costs do apply compared to the standard method, because we increase the scale in order to obtain the guaranteed yield. Without dsRNA reduction, protein expression is typically 10-fold lower than with dsRNA reduction.

    Uncapped, triphosphorylated RNA

    Secondly, currently no supplier can promise that 100% of all mRNA molecules will be capped. When uncapped, the 5’UTR of the mRNA contains a 5′ triphosphate, which can be a ligand for the cytoplasmic innate immune sensor RIG-I. RiboPro offers 2 capping grades (~80% and 95%+). If selecting the lower grade (cheaper), additional phosphatase treatment may be required to reduce RIG-I activation.

    Cap methylation status

    Third, if a cap is present, but no additional methylation of the first nucleotide (Cap0), then IFIT1 may be activated in certain cells, leading to around 30-50% reduction in activity in some experiments. This seems to be particularly relevant in cells of the immune system. RiboPro offers optional methylation of the first nucleotide (Cap1).

    Contaminants

    Finally, template DNA, salts and small RNA species (formed during abortive cycling of the RNA polymerase) must be carefully removed to avoid interfering with uptake and interpretation of results. RiboPro uses extensive DNAse I-based DNA removal, reducing the residual DNA to far less than 1 millionth of the RNA concentration.

    QC

    Next to an optimized production process, RiboPro also has also developed a series of test for quality control, offering you peace of mind about the identity, purity and modification status of your order.

    Do you have additional questions? Please contact us directly below or later via the contact page. We are happy to help!