Custom mRNA

Built on more than 10 years of experience in mRNA synthesis, we are your trust-worthy source of custom mRNA. From µg-scale up to 100 grams, and lengths up to 15knt, we have you covered.

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  • Highest quality mRNA
  • Fastest turn-around
  • Support for success
  • Sensible scales

Custom mRNA synthesis

Our custom mRNA service produces high quality mRNA based on custom sequence designs, which may originate from various sources:

  1. your own sequence design, manufactured exactly according to your specification
  2. your sequence, optimized with our technologies for optimal expression
  3. a sequence designed by us, based on the protein you like to express.

Our fully enzymatic process is able to use several types of DNA as input, including:

  1. circular DNA plasmid provided by you or your DNA supplier
  2. circular DNA plasmid cloned by RIBOPRO
  3. linear DNA from an de novo DNA synthesis, and/or PCR method

 

Choices

When ordering custom mRNA you have various choices and options to customize the product to your exact needs. Is your favorite option not listed? Please contact us and we are happy to discuss a custom project with you.

Choice Description
Capping Co-transcriptional (ARCA)
Enzymatic (VCE/FCE)
Capping efficiency 80-95% (ARCA)
95-99% (VCE/FCE)
Cap-type Cap-0 (for standard cell-types)
Cap-1 (for immune cells & in vivo applications)
Poly(A)tail length 0-300nt
Segmented
Amount 100 µg – 100 g
Buffer PBS (pH 7.4)
Citrate (pH 5.5)
Other buffer

How to get a quote?

Tells us via our Quotation form exactly what you want and you will receive an initial quotation as soon as possible. In the form you can also add questions, remarks, and ask our RiboProfessionals™ to contact you to help make adjustments to your mRNA-design/mRNA-project. Filling out the form is fast, easy and prepares our people to support you the best they can!

Get a quote Schedule a meeting

What will happen next?

After your quotation/order request

Once you send the form in, we take the following steps a.s.a.p. to help you get the best mRNA as fast as possible:
1. a RiboProfessional™ checks your request,
2. gets in touch if needed, and
3. sends you a quotation within 24h
4. when you OK the quotation, we get started.

For repeat orders (an option in our ordering menu), you will still get a quotation, but we often already start with synthesis ‘at risk’ from DNA that is ‘on file’ to realize the shortest possible turn-around time. Of course, you may still ask for amendments of the order based on the quotation. The risk is entirely ours.

After your order

During production, we do the following:

  1. Optimize the sequence if desired
  2. Communicate the optimized sequences back to you (optional)
  3. Order DNA if needed
  4. Perform RNA synthesis
  5. Perform Quality Control
  6. Arrange shipping
  7. Check if material has arrived complete and correct
  8. Send invoice
  9. Follow-up to help you with your experiments if needed

For planning purposes, you will receive updates at steps 2, 4 and 6.

Custom fluorescent mRNA synthesis

If you need a special mRNA sequence with fluorescent-labelling or if you need a special label itself, then you may order custom mRNA with a pre-defined degree of mRNA labelling.

Our custom mRNA service produces high quality mRNA based on custom sequence designs, which may originate from various sources:

  1. your own sequence design, manufactured exactly according to your specification
  2. your sequence, optimized with our technologies for optimal expression
  3. a sequence designed by us, based on the protein you like to express.

Our fully enzymatic process is able to use several types of DNA as input, including:

  1. circular DNA plasmid provided by you or your DNA supplier
  2. circular DNA plasmid cloned by RIBOPRO
  3. linear DNA from an de novo DNA synthesis, and/or PCR method

We synthesize the mRNA with a predefined percentage of  click-chemistry-enabled nucleotides, allowing the incorporation of a wide variety of dyes evenly-distributed over the length of the mRNA. Importantly, fluorescent-labelling of the mRNA will reduce the translational activity proportionally with the labelling degree.

Choices

When ordering custom mRNA you have various choices and options to customize the product to your exact needs. Is your favorite option not listed? Please contact us and we are happy to discuss a custom project with you.

Choice Description
Label AF647 (ex 647nm / em 670nm)
AF568 (ex 568nm / em 603nm)
AF488 (ex 488nm / em 530nm)
Other dyes available upon request
Capping Co-transcriptional (ARCA)
Enzymatic (VCE/FCE)
Capping efficiency 80-95% (ARCA)
95-99% (VCE/FCE)
Cap-type Cap-0 (for standard cell-types)
Cap-1 (for immune cells & in vivo applications)
Poly(A)tail length 0-300nt
Segmented
Amount 100 µg – 100 g
Buffer PBS (pH 7.4)
Citrate (pH 5.5)
Other buffer

How to get a quote?

Tells us via our Quotation form exactly what you want and you will receive an initial quotation as soon as possible. In the form you can also add questions, remarks, and ask our RiboProfessionals™ to contact you to help make adjustments to your mRNA-design/mRNA-project. Filling out the form is fast, easy, and prepares our people to support you the best they can!

Get a quote Schedule a meeting

What will happen next?

After your quotation/order request

Once you send the form in, we take the following steps a.s.a.p. to help you get the best mRNA as fast as possible:
1. a RiboProfessional™ checks your request,
2. gets in touch if needed, and
3. sends you a quotation within 24h
4. when you OK the quotation, we get started.

For repeat orders (an option in our ordering menu), you will still get a quotation, but we often already start with synthesis ‘at risk’ from DNA that is ‘on file’ to realize the shortest possible turn-around time. Of course, you may still ask for amendments of the order based on the quotation. The risk is entirely ours.

After your order

During production, we do the following:

  1. Optimize the sequence if desired
  2. Communicate the optimized sequences back to you (optional)
  3. Order DNA if needed
  4. Perform RNA synthesis
  5. Perform Quality Control
  6. Arrange shipping
  7. Check if material has arrived complete and correct
  8. Send invoice
  9. Follow-up to help you with your experiments if needed

For planning purposes, you will receive updates at steps 2, 4 and 6.

Request quote

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Custom mRNA
DNA Template*
Sequence optimization*
Purpose*

Labelled*

Per default capping is based on ARCA Active. Other capping systems (like Vaccinia) are available on request. Cap 1 is advised, unless application type is immortalized cells, then Cap 0 is advised.
5 'UTR*

3 'UTR*

Poly A tail*

Solvent*

Welcome on our new website

Please bear with us while we get everything in order.
Importantly, we are ready to receive your order, quote request or meeting request as per usual.
We look forward helping you with the highest quality mRNA.