RiboPro provides custom LNP formulation services for research and therapeutic applications.
Whether your RNA is manufactured by RiboPro or supplied externally, we can formulate it using customer-provided lipids or our proprietary lipid platform developed for efficient delivery and low toxicity
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RiboPro provides custom lipid nanoparticle (LNP) formulation services for mRNA and other RNA-based therapeutics. We can formulate both RiboPro-produced RNA and customer-supplied RNA using either customer-provided lipids or our proprietary lipid systems.
Our LNP platform combines proprietary ionizable and core lipid technologies with an in-house developed microfluidic formulation process. The result is highly reproducible LNP formulations designed to achieve efficient RNA delivery while maintaining low toxicity and low immunogenicity.
A key differentiator of our platform is the use of a proprietary core lipid that demonstrates reduced liver biodistribution compared with conventional cholesterol-based formulations, creating opportunities for delivery beyond the liver. Combined with efficient endosomal escape, our LNPs are well suited for advanced research and therapeutic development applications.
Typical formulation characteristics:
| Parameter | Typical Value |
| Particle size | 50-200 nm |
| PDI | < 0.2 |
| Encapsulation Efficiency | > 85% |
| RNA concentration | 0.01-0.5 mg/mL |
Our services include:
Every RNA program has unique delivery requirements. Our formulation services can be tailored to your application, target tissue, analytical requirements, and downstream development goals.
| Customization Option | Available Options |
| Formulation | Standard and proprietary LNP formulations tailored to your application. |
| Target delivery | Co-development of targeted LNP systems using our proprietary lipid technologies. |
| Buffer | Standard buffer: TBS + 10% sucrose, optimized for frozen storage and transport. Alternative buffer systems available upon request. |
| Sterile filtering | 0.22 μm 0.45 μm |
| QC assays | Particle size and PDI by DLS Zeta potential RNA encapsulation by RiboGreen Apparent pKa by TNS (upon request) |
RiboPro LNPs delivered eGFP-encoding mRNA to cultured cells, resulting in strong and widespread reporter expression. Under the tested in vitro conditions, RiboPro LNPs produced cellular eGFP fluorescence within the range of the benchmark LNP and a higher average signal than the third-party formulation.
Fluorescence microscopy confirms eGFP expression across the cell population, demonstrating that the delivered mRNA remained functional and was successfully translated by the cells.
Cells were imaged and analyzed 24 hours after treatment. Cellular eGFP fluorescence was quantified using corrected total cellular fluorescence (CTCF). Representative fluorescence, brightfield and merged microscopy images are shown at low and high magnification. Scale bars represent 50 µm.
RiboPro LNPs have been used for delivery of mRNA cancer vaccine candidates designed to enhance antigen-specific T-cell responses. In preclinical studies, LNP-formulated mRNA constructs generated strong IFNγ responses and were associated with reduced tumour growth.
The data illustrate an example of the delivery of HPV E7-OligoDOM™ mRNA using the RiboPro LNP platform. In a prophylactic TC-1 tumour model, mice were immunized prior to tumour challenge (A). Analysis of splenocytes showed strong antigen-specific IFNγ responses following vaccination, indicating effective activation of T cells against the tumour-associated antigen (B). Importantly, these enhanced immune responses translated into reduced tumour growth compared with control groups, with complete tumour growth suppression observed in the 2 µg HPV E7-OligoDOM™ treatment group (C).
These results demonstrate the ability of the RiboPro LNP platform to efficiently deliver mRNA cancer vaccine candidates and support the generation of potent anti-tumour immune responses.
Further details on the experimental setup and results can be found in the full study.
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