Products & Services

Based on deep knowledge, we offer a suite of services that help innovators create the medicines of the future.

Our one-stop-shop has you covered on:

  • mRNA design
  • R&D- and GMP-grade manufacturing
  • mRNA- and delivery-focussed contract research
  • Experiment and clinical trial design
  • Bi-directional tech-transfer
  • Licensing

Optimal performance

mRNA/therapy design

The performance of an mRNA for research, vaccine or therapeutic use is critically dependent on several factors, including:
– the choice of therapeutic target (variant),
– the design of the sequence,
– (chemical) modifications,
– delivery vehicle composition, and
– targeting strategy

The combinatorial effects of these parameters can make selecting the most optimal composition challenging. Luckily, RIBOPRO has the experience and tools to help you design the best mRNA/product for your application.

  • Sequence optimisation

    RIBOPRO offers the design of your RNA product using proprietary algorithmns to optimize sequences for various purposes.

    These algorithmns use a combination of:

    our patented sequence-based de-immunization technology, ensuring optimal protein expression by avoiding ribosome inhibition as the consequence of TLR7/8 and TLR3 activation by the mRNA;
    codon-optimization to improve protein expression in the target tissue based on the cell-type specific abundance of tRNAs, codon usage and protein-folding kinetics; and
    proprietary 5′ and 3’UTR libraries that can be combined to achieve the desired Ribosome-loading activity (5’UTR) and stability (3’UTR). Our UTRs are synthetic in origin and designed to add a minimum of weight to the product, maximizing the amount of gene product delivered per dose;

    to generate an mRNA with the desired safety and expression profile.

  • Formulation design

    Based on our patent-pending class of ionizable lipids, and novel core-lipid construct, we can design LNP-formulations for various types of applications. We have lipids available that are suitable for vaccination by delivering an adjuvating signal, as well as lipids with very low immunogenicity for therapy development.

     

    However, we are not restricted to using our proprietary lipids. We also have extensive experience with formulating mRNA with commercially available lipids and experimental customer-generated lipids.

     

    Working together with the targeting experts at mRNA drug-developer Mercurna, we offer tissue- or cell-specific targeting modifications of the LNP. A limited number of peptide-based targeting moeities is already available and via co-development novel targeting peptides may be developed. Non-peptide targeting ligands such as antibodies, DARPINs, glycans and others are also possible, because we have a variety of click chemistries available.

  • Experiment/trial design

    Beyond the design of your product, we use our extensive experience with literally hundreds of customer projects to help you get the most out of your studies through protocols, advice, and data interpretation.

     

    We call that our success service, and it is included free-of-charge with any other product of service.

From R&D to GMP

Manufacturing services

Reproducibly producing high quality mRNA in sufficient amount is not easy and requires tight control over the process parameters, a stringent quality control and the use of high quality raw materials.
RIBOPRO uses highly innovative, proprietary methods to synthesize high-quality, high-purity full-length mRNA, with low levels of dsRNA, high capping efficiency and well-defined poly(A)-tails, from either de novo or customer delivered DNA templates.

As a critical benefit for drug-developers, our process is highly scalable and GMP-ready. Especially now our GMP-facility is coming online in Q1 of 2023, choosing RIBOPRO means seamless continuity from R&D to GMP.

  • High purity mRNA

    RiboPro has developed a proprietary system using in-house developed enzymes with enhanced stability, activity and fidelity combined with progressively optimized, automated protocols to ensure efficient synthesis of pure, full-length mRNA.

     

    These protocols allow for the production of short (around 100nt) to extremely long (15k nt) RNA transcripts with a minimum of side-products and contaminants. Our (m)RNA products are suitable for the expression of small peptides and large proteins, the formation of circular RNA (circRNA), and as source of self-amplifying RNA (saRNA).

     

    Any residual impurities are removed by our purification system, reducing such impurities at least 1000-fold.

  • Complete capping

    To turn the basic RNA transcript into a fully functional mRNA, capping (addition of a 5′ cap) and A-tailing is required. Completeness of capping is not only required for full activity, but also to avoid innate immunity mediated by for example RIG-I.

     

    We currently offer 2 methods of capping:

    1. with our enzymatic Vaccinia capping system >99% capped mRNA can be achieved for typical, uncomplicated 5’UTRs,
    2. with our ARCA (Anti-reverse cap analog) or another cap analog based method complicated 5’UTRs (with major or very stable secondary structure) can be capped co-transcriptionally. Of note, RIBOPRO has developed a proprietary method (ARCA-Active™) to ensure >95% capping with ARCA (100% correct orientation), where traditional protocols provide 50-80% capping via ARCA.
    Of course, in case of customer preference, also uncomplicated 5’UTRs can be capped using the co-transcriptional ARCA-based method.

  • Awesome A-tailing

    For the addition of the poly(A)-tail, we offer currently 3 methods:

    1. PCR-based addition to the DNA template as a rapid and robust method of ensuring >99% of RNA molecules carry an A-tail of sufficient length.
    2. an enzymatic method producing a narrow distribution of fully-customizable A-tail lengths, and
    3. a plasmid template-based method producing a highly defined A-tail length. Frequently requested lengths (120/150nt) can be provided from stock templates.

    Having high quality, well-defined poly(A)-tail ensure reliable expression over a defined period of time.

Shop for custom mRNA

Deep dive into GMP mfg.

Custom RNA

Starting from €600

For research & development

  • For research & development
  • Our experts help design your mRNA if desired
  • Any length sequence to 15k nt (longer upon request)
  • Fast delivery
Choose product
Read more about Custom mRNA
Off-the-shelf reporter mRNA

Starting from €250

Affordable & ready-to-use

  • Directly available off-the-shelf
  • Verified to work
  • Perfect as control or to prove delivery
  • Most affordable options
Choose product
Read more about Off-the-shelf mRNA

The right tools, attention and the best ingredients inspire to realize even the biggest dreams.

Sander van AsbeckCEO / Founder

Fantastic formulations

Formulation services

Any mRNA purchased from RiboPro can be formulated into a selection of nanoparticle technologies.

These technologies include:
– cationic peptides,
– cationic or ionizable polymers,
– cationic or ionizable lipids.

Our specialty is formulation in lipid nanoparticles (LNPs) of which we have several standard formulations, based on proprietary lipids, available that display high mRNA delivery efficiency with low toxicity and immunogenicity.

  • Service content

    Our formulation services include:
    – Producing nanoparticles of defined size
    (80-250nm, PDI <0.02)
    – Buffer exchange (via dialysis)
    – Dilution or concentration
    (0.01-2mg/ml mRNA, standard 1mg/ml)
    – Quality control (Size by DLS)
    – Packaging and shipping

     

    You may choose from various options:
    Buffer
    – Phosphate pH6-8, standard pH7.4
    – Citrate pH4-6
    – MES pH5.5-6.5
    – HEPES pH7-8
    – Others upon request
    Sterile filtering
    – 0.22um (recommended)
    – 0.45um
    QC-assays:
    – Zeta-potential by DLS
    – Surface-charge by TNS
    – RNA-encapsulation by RiboGreen

     

    NB. We do not formulate mRNA obtained elsewhere.

mRNA focussed

Contract Research

Not everyone has access to a lab or the time to do basic mRNA testing. Or, you may lack mRNA-specific knowledge or equipment. When ordering mRNA from RiboPro, you don’t have to have any of that; you have access to RiboPro’s CRO services. We offer a variety of standardized assays to assess the activity, toxicity and/or immunogenicity from your mRNA and/or LNP formulation.

This service is ideal for virtual biotechs and other that want to make rapid advances in the mRNA space.

All our CRO services are executed by experienced scientists.

  • Service content

    RiboPro offers the following mRNA-focused (standard) CRO services:

    – mRNA activity testing
    – Protein expression profiling
    – Immunological activation testing
    – Toxicity testing (in vitro only)
    – Release from bio-material testing

     

    Other tests/assays may be available upon discussion. Contact our RiboProfessionals to learn what we can do for you!

    Schedule a meeting

Combining our services puts your project on the short-track to success

Sander van AsbeckCEO / Founder

Superior outcomes

Success services

Because RIBOPRO is a mission-driven company we care about your success, whether that is the succesful answer of a research question, or the development of a new medicine. Therefore, we are always available for advice, data-interpretation, and/or experiment/trial design, free-of-charge.

So, please do not hesitate to contact one of our RiboProfessionals to help you accomplish more.

Deep dive into our success services

Customers

What our customers say

“We are RiboPro customer since 2020 and we are satisfied about their products. We also appreciate very much the customer service. The team is very cooperative and helpful, communication is clear, precise and always on time.”

Chiara Gai

“I ordered a custom Cap-1 mRNA from RiboPro for in vivo research. The custom mRNA from RiboPro expressed as the target protein in the mammalian cell line and maintained good level of protein expression in vivo (mouse). They delivered me the mRNA product in a timely manner and gave useful comments, responses and transparency to my questions for my order. So, I would recommend users trying the RiboPro.”

Lee Jenny

“RiboPro offers not only high-quality mRNA but also very helpful and fast support on all matters concerning mRNA.”

Jana Schembera

Researcher

“The high quality, stable and non-immunogenic mRNA generated by Ribopro is extremely suitable to meet our needs. The company also offers the qualified expert advice allowing the optimization of our experimental protocols.”

Prof. Dr. Elena Levtchenko

Professor at KU Leuven

We take the work out of designing your mRNA so you can accomplish more.

Schedule a meeting

Welcome on our new website

Please bear with us while we get everything in order.
Importantly, we are ready to receive your order, quote request or meeting request as per usual.
We look forward helping you with the highest quality mRNA.