Custom mRNA synthesis

500.00 425.00

RiboPro offers custom mRNA synthesis, so you can get started with your experiment as quickly as possible, without hassle!

No need to worry about RNAse contamination during production, no need to setup complex quality control, no need to invest in the equipment and materials; we take care of it all.

We offer 2 easy ways to order custom mRNA: if you want to be helped by an RNA expert, you can send us a message via this contact form and our sales representatives will contact you to help you with every step of the way. Or you can use the mRNAssembler™ below to design your mRNA, instantly see the price and simply add the designed sequence to your cart to order.

For a typical sequence, you will receive your order in 2-3 weeks.

Do you want to know more before making a decision? Scroll down for additional info and reviews at the bottom of this page.


mRNAssembler™ – quick and easy ordering of your mRNA

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Description

The performance of a messenger RNA, to be added to cells via standard transfection methods, greatly depends on the design of the sequence, the quality of the synthesis, chemical modifications of specific bases and the purity of the end-product. Introducing a sub-optimal mRNA into cells is known to trigger the innate immune response; resulting in lower protein expression, cellular toxicity and the release of pro-inflammatory cytokines.

For the best performance, we recommend the following combination of features to be selected for your custom mRNA:

  • 5’UTR: no upstream ATG, kozak or similar present directly upstream of the startcodon. For example RiboPro catalogue 5’UTRs.
  • 3’UTR: selected from mRNAs known to be extra-ordinary stable.
  • Cap1 – 95%.
  • 120-150nt A-tail.
  • dsRNA reduction.

If you like to understand why these settings lead to the best performance, read more below.

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Synthesizing a high-performance messenger RNA requires optimization all through-out the multi-step synthesis, including specialized purification.
First of all, during the enzymatic production process, dsRNA can be formed. dsRNA is one of the main triggers of innate immunity, primarily via TLR3 (endosomal recognition during transfection), OAS1, PKR and MDA5 (cytoplasmic sensors). RiboPro’s mRNA synthesis process results in the lowest amount of dsRNA possible. The method used reduces the yield of single synthesis, so additional costs (limited amount) do apply compared to the standard method, because we increase the scale in order to obtain the guaranteed yield. Without dsRNA reduction, protein expression is typically 10-fold lower than with dsRNA reduction.
Secondly, currently no supplier can promise that 100% of all mRNA molecules will be capped. When uncapped, the 5’UTR of the mRNA contains a 5′ triphosphate, which can be a ligand for the cytoplasmic innate immune sensor RIG-I. RiboPro offers 2 capping grades (~80% and 95%+). If selecting the lower grade (cheaper), additional phosphatase treatment may be required to reduce RIG-I activation.
Third, if a cap is present, but no additional methylation of the first nucleotide (Cap0), then IFIT1 may be activated, leading to around 30-50% reduction in activity in some experiments. RiboPro offers optional methylation of the first nucleotide (Cap1).
Finally, removal of template DNA, salts and small RNA species (formed during abortive cycling of the RNA polymerase) must be carefully removed to avoid interfering with uptake and interpretation of results.

Next to an optimized production process, RiboPro also has also developed a series of test for quality control, offering you peace of mind about the identity, purity and modification status of your order.

Do you have additional questions? Please contact us below or later via the contact page. We are happy to help!

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Additional information

Scale

Standard scales from 100µg to 10mg or Custom scales from 10mg

Templates

De novo synthesis or customer supplied

UTRs

Customer supplied or RiboPro catalogue

Modifications

Cap0 or Cap1. Phosphatase treatment, dsRNA reduction.

A-tailing

Enzymatic or Primer-based. All lengths from 3-1000nt

Quality control

Length, Purity, Quantity, A-tailing, Capping %, dsRNA %

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