Custom mRNA synthesis

A complete product from 500.00 425.00

RiboPro offers custom mRNA synthesis, so you can get started with your experiment as quickly as possible, without hassle!

No need to worry about RNAse contamination during production, no need to setup complex quality control, no need to invest in the equipment and materials; we take care of it all.

We offer 2 easy ways to order custom mRNA: if you want to be helped by an RNA expert, you can send us a message via this contact form and our sales representatives will contact you to help you with every step of the way. Or you can use the mRNAssembler™ below to design your mRNA, instantly see the price and simply add the designed sequence to your cart to order.

For a typical sequence, you will receive your order in 2-3 weeks.

Do you want to know more before making a decision? Scroll down for additional info and reviews at the bottom of this page.


mRNAssembler™ – quick and easy ordering of your mRNA

Transcript 1

In the menu below, please select the properties you require for the first mRNA of your order. You will be able to add more mRNAs to your order in the tabs below.

  • Step 1/4 - Name your sequence
    Please provide a unique identifier that will be printed on your tube.
  • Min: 3 charactersMax: 50 characters

  • Step 2/4 - Select a template for RNA synthesis
    mRNA synthesis requires a DNA template. Our process uses the T7 phage RNA polymerase and is able to handle de novo synthesized templates and cloned plasmid templates. If you already have a construct of the sequence of interest, you may upload the sequence and we will review its suitability. If suitable, you will receive a kit with instructions for packaging and sending an aliquot to be used as template during synthesis.
  • Step 3/4 - Please provide some details on the sequence
    Next we require some information on the sequence to determine the price for synthesis. You may select 5'UTR, coding sequence and 3'UTR. Additional options follow below. In any case we keep you sequence confidential.

  • Max: 3000 characters

  • Please fill your coding sequence, starting ATG and ending in a in-frame stop codon. Only use A, T, G and C; this will be converted to A, U, G and C.Min: 30 charactersMax: 6000 characters

  • 3'UTR unavailableAt the moment RiboPro does not have any catalog 3'UTR sequences, so please include your own 3'UTR in the sequence..

    At the moment RiboPro does not have any catalog 3'UTR sequences, so please fill in your own 3'UTR.

  • Please fill in a 3'UTR sequence

  • Step 3/4 - Please provide some details on the sequence
    Below we ask some details on your desired mRNA sequence. This aids us in determining the suitability of your sequence.
  • Please fill the sequence of the ENTIRE desired mRNA (5'UTR, coding sequence and 3'UTR). Only use A, T, G and C; this will be converted to A, U, G and C.Min: 100 charactersMax: 6000 characters

  • Step 4/4 - Please select RNA modification and purification options
  • Capping is generally required for activity of the mRNA, unless an IRES sequence or similar is present. Cap0 is regular capped and Cap1 has additional methylation of the first nucleotide following the cap. Cap1 has been found to increase expression up to 50% in specific conditions and represents the more natural cap.

  • Optional phosphatase treatment to remove the triphosphate on the 5'end of the mRNA molecules that have been missed by the capping procedure. Removal of 5' triphosphates reduces immunogenicity. Highly recommended if no capping is selected.

  • Max: 300

  • dsRNA is another important source of innate immunity, which greatly affects safety and protein expression. Learn more in our blog posts on dsRNA.

  • Shipping on dry ice incurs additional charges. The height of those charges depends on your location.

Transcript 2

In the menu below, please select the properties you require for the second mRNA of your order. You will be able to add more mRNAs to your order below.

  • A unique identifier to be printed on your tube and documentation.Min: 3 charactersMax: 50 characters

  • Please fill your coding sequence, starting ATG and ending in a in-frame stop codon. Only use A, T, G and C; this will be converted to A, U, G and C.Min: 30 charactersMax: 6000 characters

  • Your 5'UTR should start with (G)GGANA. If it does not, please add these nucleotides in front. Min: 10 charactersMax: 1000 characters

  • At the moment RiboPro does not have any catalog 3'UTR sequences, so please fill in your own 3'UTR.Min: 1 charactersMax: 3000 characters

  • Capping is generally required for activity of the mRNA, unless an IRES sequence or similar is present. Cap0 is regular capped and Cap1 has additional methylation of the first nucleotide following the cap. Cap1 has been found to increase expression up to 50% in specific conditions and represents the more natural cap.

  • Optional phosphatase treatment to remove the triphosphate on the 5'end of the mRNA molecules that have been missed by the capping procedure. Removal of 5' triphosphates reduces immunogenicity. Highly recommended if no capping is selected.

  • Max: 300

  • dsRNA is another important source of innate immunity, which greatly affects safety and protein expression. Learn more in our blog posts on dsRNA.

Transcript 3

In the menu below, please select the properties you require for the third mRNA of your order. You will be able to add more mRNAs to your order below.

  • A unique identifier to be printed on your tube and documentation.Min: 3 charactersMax: 50 characters

  • Please fill your coding sequence, starting ATG and ending in a in-frame stop codon. Only use A, T, G and C; this will be converted to A, U, G and C.Min: 30 charactersMax: 6000 characters

  • Your 5'UTR should start with (G)GGANA. If it does not, please add these nucleotides in front. Min: 10 charactersMax: 1000 characters

  • At the moment RiboPro does not have any catalog 3'UTR sequences, so please fill in your own 3'UTR.Min: 1 charactersMax: 3000 characters

  • Capping is generally required for activity of the mRNA, unless an IRES sequence or similar is present. Cap0 is regular capped and Cap1 has additional methylation of the first nucleotide following the cap. Cap1 has been found to increase expression up to 50% in specific conditions and represents the more natural cap.

  • Optional phosphatase treatment to remove the triphosphate on the 5'end of the mRNA molecules that have been missed by the capping procedure. Removal of 5' triphosphates reduces immunogenicity. Highly recommended if no capping is selected.

  • Max: 300

  • dsRNA is another important source of innate immunity, which greatly affects safety and protein expression. Learn more in our blog posts on dsRNA.

Transcript 4

In the menu below, please select the properties you require for the fourth mRNA of your order. You will be able to add more mRNAs to your order by adding these 4 to your cart and then revisiting this page. For bulk ordering please contact our sales team for a excel template.

  • A unique identifier to be printed on your tube and documentation.Min: 3 charactersMax: 50 characters

  • Please fill your coding sequence, starting ATG and ending in a in-frame stop codon. Only use A, T, G and C; this will be converted to A, U, G and C.Min: 30 charactersMax: 6000 characters

  • Your 5'UTR should start with (G)GGANA. If it does not, please add these nucleotides in front. Min: 10 charactersMax: 1000 characters

  • At the moment RiboPro does not have any catalog 3'UTR sequences, so please fill in your own 3'UTR.Min: 1 charactersMax: 3000 characters

  • Capping is generally required for activity of the mRNA, unless an IRES sequence or similar is present. Cap0 is regular capped and Cap1 has additional methylation of the first nucleotide following the cap. Cap1 has been found to increase expression up to 50% in specific conditions and represents the more natural cap.

  • Optional phosphatase treatment to remove the triphosphate on the 5'end of the mRNA molecules that have been missed by the capping procedure. Removal of 5' triphosphates reduces immunogenicity. Highly recommended if no capping is selected.

  • Max: 300

  • dsRNA is another important source of innate immunity, which greatly affects safety and protein expression. Learn more in our blog posts on dsRNA.

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Description

The performance of a messenger RNA, to be added to cells via standard transfection methods, greatly depends on the design of the sequence, the quality of the synthesis, chemical modifications of specific bases and the purity of the end-product. Introducing a sub-optimal mRNA into cells is known to trigger the innate immune response; resulting in lower protein expression, cellular toxicity and the release of pro-inflammatory cytokines.

For the best performance, we recommend the following combination of features to be selected for your custom mRNA:

  • 5’UTR: no upstream ATG, kozak or similar present directly upstream of the startcodon. For example RiboPro catalogue 5’UTRs.
  • 3’UTR: selected from mRNAs known to be extra-ordinary stable.
  • Cap1 – 95%.
  • 120-150nt A-tail.
  • dsRNA reduction.

If you like to understand why these settings lead to the best performance, read more below.

Show More

Synthesizing a high-performance messenger RNA requires optimization all through-out the multi-step synthesis, including specialized purification.
First of all, during the enzymatic production process, dsRNA can be formed. dsRNA is one of the main triggers of innate immunity, primarily via TLR3 (endosomal recognition during transfection), OAS1, PKR and MDA5 (cytoplasmic sensors). RiboPro’s mRNA synthesis process results in the lowest amount of dsRNA possible. The method used reduces the yield of single synthesis, so additional costs (limited amount) do apply compared to the standard method, because we increase the scale in order to obtain the guaranteed yield. Without dsRNA reduction, protein expression is typically 10-fold lower than with dsRNA reduction.
Secondly, currently no supplier can promise that 100% of all mRNA molecules will be capped. When uncapped, the 5’UTR of the mRNA contains a 5′ triphosphate, which can be a ligand for the cytoplasmic innate immune sensor RIG-I. RiboPro offers 2 capping grades (~80% and 95%+). If selecting the lower grade (cheaper), additional phosphatase treatment may be required to reduce RIG-I activation.
Third, if a cap is present, but no additional methylation of the first nucleotide (Cap0), then IFIT1 may be activated, leading to around 30-50% reduction in activity in some experiments. RiboPro offers optional methylation of the first nucleotide (Cap1).
Finally, removal of template DNA, salts and small RNA species (formed during abortive cycling of the RNA polymerase) must be carefully removed to avoid interfering with uptake and interpretation of results.

Next to an optimized production process, RiboPro also has also developed a series of test for quality control, offering you peace of mind about the identity, purity and modification status of your order.

Do you have additional questions? Please contact us below or later via the contact page. We are happy to help!

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Additional information

Scale

Standard scales from 100µg to 10mg or Custom scales from 10mg

Templates

De novo synthesis or customer supplied

UTRs

Customer supplied or RiboPro catalogue

Modifications

Cap0 or Cap1. Phosphatase treatment, dsRNA reduction.

A-tailing

Enzymatic or Primer-based. All lengths from 3-1000nt

Quality control

Length, Purity, Quantity, A-tailing, Capping %, dsRNA %

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