The performance of a messenger RNA, to be added to cells via standard transfection methods, greatly depends on the design of the sequence, the quality of the synthesis, chemical modifications of specific bases and the purity of the end-product. Introducing a sub-optimal mRNA into cells is known to trigger the innate immune response; resulting in lower protein expression, cellular toxicity and the release of pro-inflammatory cytokines.
For the best performance, we recommend the following combination of features to be selected for your custom mRNA:
- 5’UTR: no upstream ATG, kozak or similar present directly upstream of the startcodon. For example RiboPro catalogue 5’UTRs.
- 3’UTR: selected from mRNAs known to be extra-ordinary stable.
- Cap1 – 95%.
- 120-150nt A-tail.
- dsRNA reduction.
If you like to understand why these settings lead to the best performance, read more below.Show More
Do you have additional questions? Please contact us below or later via the contact page. We are happy to help!Show More