The performance of a long non-coding RNA, to be added to cells via standard transfection methods, differs from messenger RNA; there is no need for protein production. However, similar to in vitro transcribed (IVT) messenger RNA, IVT long non-coding RNA can trigger unwanted innate immunogenicity, effecting cell-wide transcription levels, potentially affecting experimental readout. These potential side-effects greatly depends on the design of the sequence, the quality of the synthesis, chemical modifications of specific bases and the purity of the end-product.
For the best performance, we recommend the following combination of features to be selected for your custom lncRNA (if lncRNA is endogenously synthesized by RNApol II and bears a cap and A-tail):
- Cap1 – 95%.
- 120-150nt A-tail.
- dsRNA reduction.
- optional pre-folding by rapid or slow cooling after heating.
If you like to understand why these settings lead to the best performance, read more below.Show More
Do you have additional questions? Please contact us below or later via the contact page. We are happy to help!Show More